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A new insight into isotopic fractionation associated with decarboxylation in organisms: implications for amino acid isotope approaches in biogeoscience

Abstract

Stable nitrogen (15N/14N) and carbon (13C/12C) isotopic compositions of amino acids in organisms have widely been employed as a powerful tool to evaluate resource utilization and trophic connection among organisms in diverse ecosystems. However, little is known about the physiological factors or mechanisms responsible for determining the isotopic discrimination (particularly for carbon) within amino acids of organisms. In the present study, we investigated the inter-trophic discrimination of nitrogen and carbon isotopes within amino acids (Δδ15NAA and Δδ13CAA, respectively) using four consumer–diet pairs. Each pairing illustrates a metabolic perspective of isotopic fractionation of amino acids. The Δδ15NAA values in these combinations reveal a trend consistent with those observed in many other combinations in previous studies. This further validates a standard scenario: the deamination preferentially removes 14N amino group from diet-derived amino acids, leaving behind the 15N-enriched amino acids in consumer biomass. The Δδ15NAA values thus mirror the activity of amino acid deamination in consumers. In contrast, the trends in the Δδ13CAA value suggest a different metabolic fate for the amino acid carbon isotope. Based on our results, we predict the following scenario: decarboxylation preferentially removes 12C α-carbon (i.e., carbonyl-carbon) from pyruvic acid in glycolysis, and from α-ketoglutaric acid in the tricarboxylic acid cycle, leaving behind the 13C-enriched both pyruvic and α-ketoglutaric acids. The 13C is then transferred to amino acids that are synthesized from the 13C-enriched precursor molecules within consumers. The Δδ13CAA values therefore mirror the pathways of de novo amino acid synthesis in consumers. The proposed link between nitrogen and carbon isotopes can refine our knowledge of the potential processes affecting the isotopic fractionation within diet and consumer compartments, as well as environmental samples.

Graphical abstract

Definition and Equations

δ15N, stable isotopic composition of nitrogen;

$$ {\delta}^{15}\mathrm{N}\kern0.20em (permil)=\left(\left({}^{15}\mathrm{N}/{}^{14}{\mathrm{N}}_{\mathrm{Sample}}\right)/\left({}^{15}\mathrm{N}/{}^{14}{\mathrm{N}}_{\mathrm{AIR}}\right)-1\right)\times 1000 $$

δ13C, stable isotopic composition of carbon;

$$ {\delta}^{13}\mathrm{C}\kern0.20em (permil)=\left(\left({}^{13}\mathrm{C}/{}^{12}{\mathrm{C}}_{\mathrm{Sample}}\right)/\left({}^{13}\mathrm{C}/{}^{12}{\mathrm{C}}_{\mathrm{VPDB}}\right)-1\right)\times 1000 $$

Introduction

During the past four decades, the analysis of stable isotope ratios (e.g., 15N/14N, 13C/12C) has been used widely in the study of biogeosciences (e.g., Fry 2006). While DNA analysis can visualize the biosphere in terms of species through genetic profiles, isotope analysis can visualize the biosphere in terms of the quantitative flux in physical and/or biochemical processes (e.g., Carreon-Martinez and Heath 2010; Maeda et al. 2012; Traugott et al. 2013). Isotopic discrimination is frequently quantified by the kinetic isotope effect (α) and flux (F) of the processes (e.g., Hoering 1957; Mariotti et al. 1981; Goto et al. 2018). The wide array of biochemical reactions within organisms creates diverse and distinctive isotopic compositions within the biosphere (δ15N, ‰ vs. atmospheric nitrogen gas [AIR]; δ13C, ‰ vs. Vienna Peedee Belemnite [VPDB]). Often, these stable isotopic compositions represent discernible ratios and thus can be useful for characterizing and quantifying any given process in the biosphere (e.g., Hayes 2001; Chikaraishi 2014; Ohkouchi et al. 2015). Identification of key processes driving isotopic fractionation (ε) is therefore indispensable for a fundamental understanding of “universality” of the isotopic discrimination (Δ = δAδB; where organism A is consumer and B is diet), as well as for validating the accuracy and precision of isotopic evidence in application studies (e.g., Hayes 2001; Wada et al. 2013; Ohkouchi et al. 2015).

Nitrogen isotopes with amino acids undergo varying degrees of isotopic discrimination (Δδ15NAA) during trophic transfer (e.g., Gaebler et al. 1963, 1966; Hare et al. 1991; McClelland and Montoya 2002; Chikaraishi et al. 2007; McCarthy et al. 2007; Popp et al. 2007; Steffan et al. 2015; McMahon and McCarthy 2016; Ishikawa 2018). The patterns of Δδ15NAA values have been frequently associated with the trophic tendencies and amino acid degradation pathways within organisms (Takizawa and Chikaraishi 2014, 2017; Takizawa et al. 2017; Choi et al. 2018; Goto et al. 2018). Chikaraishi et al. (2007) proposed that deamination (preceding transamination) is a key process for isotopic fractionation of nitrogen within amino acids, which preferentially removes the 14N amino group of diet-derived amino acids and leaves behind 15N (by up to ~ 3–8‰ per trophic transfer) in the residual pool of amino acids in consumer biomass. The degree of deamination activity, therefore, results in a great diversity in the isotopic discrimination–large values for some amino acids (e.g., alanine, valine, isoleucine, proline, and glutamic acid) and small values for some other amino acids (e.g., methionine and phenylalanine) between consumer and diet-resource species, as shown in 15N-enrichment factors (Chikaraishi et al. 2009). These two groups of amino acids are frequently called “trophic” and “source” amino acids, respectively (e.g., Popp et al. 2007; reviewed in Ohkouchi et al. 2017). Using this framework, the δ15N analysis of amino acids has rapidly evolved into a unified approach: the δ15N values of the source amino acids in organisms directly provide the δ15N values of primary producers at bases of food webs, whereas difference in the Δδ15NAA value between trophic and source amino acids within a single organism correlates with its position in the trophic hierarchy (e.g., Chikaraishi et al. 2009; Steffan et al. 2013; Chikaraishi et al. 2014; Broek and McCarthy 2015; Choy et al. 2015; Lorrain et al. 2015; Blanke et al. 2017; Choi et al. 2017; Dharampal and Findlay 2017; Steffan et al. 2017; Ostrom et al. 2017; Blanke et al. 2018; Morra et al. 2019).

The large diversity in the carbon isotopic composition of amino acids (δ13CAA) has been useful in describing metabolic and trophic interactions (e.g., Abelson and Hoering 1961; Macko et al. 1987; Ostrom et al. 1994; Fantle et al. 1999; O’Brien et al. 2002; McCarthy et al. 2004; Larsen et al. 2009, 2013; McMahon et al. 2010, 2015; Fry et al. 2018; Takano et al. 2018). Trophic discrimination of carbon isotopes within amino acids (Δδ13CAA) is reportedly small or negligible for aromatic (e.g., phenylalanine) and branched-chain amino acids (e.g., valine, leucine, and isoleucine), but can vary widely between − 10‰ and + 10‰ for other amino acids (e.g., glycine, alanine, aspartic acid, proline, and glutamic acid) (Fantle et al. 1999; Howland et al. 2003; McMahon et al. 2010, 2015). Although there is little knowledge regarding the key metabolic processes responsible for the observed Δδ13CAA values (Hayes 2001; Takano et al. 2018), these two types of amino acids are not consistent with the “trophic” and “source” classification of amino acid nitrogen isotopes. Based on this dichotomy, it has been suggested that the limited amounts of de novo synthesis of essential amino acids caused the lower values of Δδ13CAA. Conversely, the large variation in the Δδ13CAA value for nonessential amino acids is attributed to frequent de novo synthesis reactions and/or metabolic routing from dietary substrates such as proteins, fats, and carbohydrates (PFC) in consumers (Fantle et al. 1999; O’Brien et al. 2002, 2005; McMahon et al. 2010; Choy et al. 2013; McMahon et al. 2015). These findings provide a framework in which the δ13C values of essential amino acids can potentially serve as a “finger-printing” tool to trace the trophic legacy of carbon from diverse dietary resources (e.g., phytoplankton, macroalgae, bacteria, and fungi) to consumer compartments (e.g., Larsen et al. 2009, 2013, 2015; McMahon et al. 2010, 2016). The identification of the key processes in organisms thus will be required for better understanding of the metabolic underpinnings of isotope ecology.

In the present study, we determined the Δδ15NAA and Δδ13CAA values together with the concentration and molar balance of amino acids in four pairs of consumer–diet invertebrates: (1) sea slug–sponge (collected from a coastal marine ecosystem), (2) ladybug beetle–aphid (collected from a terrestrial ecosystem), (3) green lacewing–fall armyworm, and (4) green lacewing–green lacewing (reared in laboratory-controlled feeding experiments). These consumer–diet pairings allow for empirical measurement of inter-trophic discrimination factor (inter-TDF) for the nitrogen and carbon isotopes (i.e., Δδ15NAA and Δδ13CAA, respectively) in a single trophic transfer, and in the process provides a mechanistic model of the isotopic fractionation processes affecting the inter-TDF of amino acids.

Materials and Methods

Natural consumer–diet combinations

The sea slug Hypselodoris festiva and the sponge Halichondria okadai were collected in May 2016, from a stony shore in Yugawara (35.080 ° N, 139.070 ° E), Japan. H. festiva is monophagous on H. okadai (e.g., Watanabe et al. 2009). Since H. okadai is ubiquitous on near-shore stones all year, H. festiva was able to feed on this diet year-round. One individual of H. festiva and one aggregation (ca. 20 mm × 50 mm) of H. okadai were collected, dried, and homogenized for the isotope analysis. We note that we did not find other individuals for H. festiva at the sampling.

Larvae of the ladybug beetle Menochilus sexmaculatus and larvae of the aphid Aphidoidea sp. were collected in September 2014, from a shrub at a house-garden in Yugawara (35.152 ° N, 139.118 ° E), Japan. M. sexmaculatus is aphidophagous in both larval and adult stages (e.g., Khan and Khan 2002), and the larvae of M. sexmaculatus collected in the present study were able to feed only on a single species of the aphid, Aphidoidea sp., within a small shrub habitat. Five individuals of M. sexmaculatus and a colony of approximately fifty Aphidoidea sp. were collected, dried, and homogenized for the isotope analysis.

Reared consumer–diet combinations

The controlled feeding experiments for the two combinations: green lacewing (Chrysoperla rufilabris)–fall armyworm (Spodoptera frugiperda) and C. rufilabrisC. rufilabris were conducted in Steffan et al. (2013). Briefly, S. frugiperda larvae were cultured on a single isotopically homogeneous diet and frozen to serve as prey for the C. rufilabris larvae. Then, a subset of the C. rufilabris larvae were frozen and used for prey for the remaining C. rufilabris larvae. Four of each trophic group (including 4–8 individuals for each trophic group) were used for the analysis. The δ15N values of amino acids were reported in Steffan et al. (2013).

Analysis of nitrogen and carbon isotopic compositions in amino acids

All samples were prepared for the isotope analysis after HCl hydrolysis and N-pivaloyl/isopropyl (Pv/iPr) derivatization, according to the procedure in Chikaraishi et al. (2009). The Pv/iPr derivatization was used for both nitrogen and carbon isotope analysis. However, for carbon isotope analysis, care was taken to keep reaction balance of pivaloyl chloride to dried sample (75 μl/mg).

The molar and isotopic compositions of amino acid derivatives were determined by gas chromatography/isotope ratio mass spectrometry (GC/IRMS) using a 6890N GC (Agilent Technologies) instrument coupled to a DeltaplusXP IRMS instrument through combustion (950 °C) and reduction (550 °C) furnaces and a countercurrent dryer (NafionTM) via a GC-C/TC III interface (Thermo Fisher Scientific). An HP Ultra-2 capillary column (50 m; i.d. 0.32 mm; film thickness 0.52 μm; Agilent Technologies) was used for chromatographic separation of amino acids. For nitrogen isotope analysis, a liquid nitrogen CO2 trap was installed between reduction and countercurrent dryer, because the generated CO2 in the combustion furnace was eliminated and the generated N2 was injected to the IRMS instrument. In contrast, for carbon isotope analysis, a liquid nitrogen CO2 trap was not installed, and the generated CO2 was injected to the IRMS instrument.

For both nitrogen and carbon, the combination of sea slug–sponge and ladybug beetle–aphid was analyzed by triplicate injections for each sample, and the combination of green lacewing–fall armyworm and green lacewing–green lacewing was analyzed by a single injection for each of four trophic groups. The δ15N and δ13C values of amino acid derivatives were obtained as the mean ± 1σ, in the triplicate injections for sea slug–sponge and ladybug beetle–aphid or in the four trophic groups for green lacewing–fall armyworm and green lacewing–green lacewing combinations. To assess the reproducibility of the isotope measurement, an isotopic reference mixture of amino acids (Indiana University; Shoko Science Co.) was analyzed after every five or six sample runs, with three pulses of reference N2 or CO2 gas discharged at the beginning and end of each run. The δ15N and δ13C values were expressed relative to the isotopic compositions of AIR and VPDB, respectively, on scales normalized to known δ values of the reference amino acids. Analytical errors for the reference mixtures were 0.3–0.6‰ (1σ for replication) for the δ15N values, and 0.7–1.4‰ (1σ for replication) for the δ13C values.

Calculation for Δδ15N and Δδ13C in amino acids

The discrimination of nitrogen isotopes (Δδ15N) of amino acids in each consumer–diet combination was calculated by the Eq. (1), because no artificial isotopic fractionation (εa) is found in experimental procedures (including derivatization) for nitrogen isotopes.

$$ \Delta {\delta}^{15}{\mathrm{N}}_{\mathrm{A}-\mathrm{B}}={\delta}^{15}{\mathrm{N}}_{\mathrm{m},\mathrm{A}}-{\delta}^{15}{\mathrm{N}}_{\mathrm{m},\mathrm{B}} $$
(1)

where δ15Nm indicates the measured δ15N value of a Pv/iPr amino acid derivative. On the other hand, the discrimination of carbon isotopes (Δδ13C) of amino acids was calculated by Eqs. (2) and (3), because artificial isotopic fractionation (εa) is considerably large in the experimental procedures (especially for the acylation) for carbon isotopes. The isotopic mass balance equation for the δ13C value of an amino acid was given by:

$$ {n}_o\times {\delta}^{13}{\mathrm{C}}_{\mathrm{o}}=\left({n}_o+{n}_d\right)\times {\delta}^{13}{\mathrm{C}}_{\mathrm{m}}-{n}_d\times \left({\delta}^{13}{\mathrm{C}}_{\mathrm{d}}-{\varepsilon}_a\right) $$
(2)

where δ13Cm and δ13Co indicate the measured and original δ13C values of an amino acid, respectively; δ13Cd and εa indicate the δ13C value of derivative groups and artificial isotopic fractionation during derivatization, respectively; and no and nd indicate the number of carbon atoms in an amino acid (e.g., two C atoms in glycine) and the derivative groups (e.g., eight C atoms in Pv/iPr glycine), respectively. The Δδ13C value was given by:

$$ \Delta {\delta}^{13}{\mathrm{C}}_{\mathrm{A}-\mathrm{B}}=\left({\delta}^{13}{\mathrm{C}}_{\mathrm{o},\mathrm{A}}-{\delta}^{13}{\mathrm{C}}_{\mathrm{o},\mathrm{B}}\right)=\left[\left({n}_o+{n}_d\ \right)/{n}_o\right]\times \left({\delta}^{13}{\mathrm{C}}_{\mathrm{m},\mathrm{A}}-{\delta}^{13}{\mathrm{C}}_{\mathrm{m},\mathrm{B}}\right) $$
(3)

Although the δ13Co values were not determined in the present study, the Δδ13C values were thus obtained by Eq. (3).

The Δδ15N and Δδ13C values were reported for seven amino acids (i.e., phenylalanine, valine, leucine, isoleucine, glycine, alanine, and glutamic acid) in the present study, because baseline separation for each peak of the amino acids was observed for these seven amino acids but not for the other amino acids (e.g., aspartic acid, proline, serine, and threonine) on the GC/IRMS chromatogram.

Results

Concentration and molar balance of amino acids

Concentration of each amino acid and molar balance among these amino acids was determined based on m/z 28 peak area of chromatograms (Table 1, Fig. 1). No substantial difference in the molar balance and concentration (weight % for total of 7 detected amino acids) is found between consumers and their diets in a given combination. We found that the molar balance in the sea slug–sponge combination is different from that in the other combinations. The former combination has a small abundance of phenylalanine (~ 6.5%) and a high abundance of glycine (~ 30%), whereas the latter combinations have a small abundance of phenylalanine (6.4–9.6%) and a high abundance of glutamic acid (~ 29%). No substantial difference (i.e., 5.2% in maximum) in the molar balance is found within a single combination, which is much smaller than the analytical error (10%) for the molar quantification of amino acids in the present study.

Table 1 Molar balance of amino acids in the combination samples
Fig. 1
figure1

Molar balance of amino acids for four combinations of consumer and diet. Error bar indicates standard deviation (1σ) of replicate measurements

Δδ15N and Δδ13C of amino acids

The Δδ15NAA values in these samples range from 0.3‰ (for phenylalanine) to 8.1‰ (for glutamic acid), and exhibit a wide diversity in their respective means and variances among the amino acids examined in the four consumer–diet pairs (Table 2, Fig. 2a). There is a low mean value (0.4‰) with little variation (0.1‰ as 1σ) for an aromatic amino acid (i.e., phenylalanine), a high mean value (from 4.0 to 5.6‰) with a little variation (from 0.7 to 1.0‰) for branched-chain amino acids (i.e., valine, leucine, isoleucine), and a large mean value (from 2.5 to 7.9‰) with large variation (from 0.2 to 2.8‰) for the other amino acids (i.e., glycine, alanine, and glutamic acid). Glycine particularly has a unique characteristic for a large variation (2.8‰) among the four combinations.

Table 2 Nitrogen and carbon isotopic discriminations on amino acids in the four combination samples
Fig. 2
figure2

a Δδ15N and b Δδ13C values of amino acids in the consumer–diet combinations for sea slug feeds on sponge (SS), ladybug beetle feeds on aphid (LA), green lacewing feeds on fall armyworm (GF), and green lacewing feeds on green lacewing (GG). Error bar indicates standard deviation (1σ) of replicate measurements

The Δδ13CAA values in these samples varies from − 1.0 (for valine and leucine) to 17.5‰ (for glycine), which is approximately twice as much as the variation in the Δδ15NAA value (Table 2, Fig. 2b). There is a low mean value (from − 0.4 to 0.1‰) with a little variation (from 0.6 to 0.9‰) for an aromatic (i.e., phenylalanine) and branched-chain (i.e., valine, leucine, and isoleucine) amino acids. On the other hand, there is a broad range of mean value (from 1.7 to 12.4‰) with variable variation (from 1.1 to 6.8‰) for the other amino acids (i.e., glycine, alanine, and glutamic acid). The highest mean value (12.4‰) with the greatest variation (6.8‰) is found for glycine among the amino acids examined in the present study.

Discussion

Analytical issues for Δδ13C of amino acids

Compound-specific isotope analysis of nitrogen and carbon within amino acids has become pervasive in many branches of science following the commercial production of the gas chromatograph-isotope ratio mass spectrometer (GC-IRMS) in the 1990s. This system has been carried out via derivatization to neutralize polar carboxyl (-COOH), amino (-NH2), and hydroxyl (-OH) groups in amino acids by less-polar moieties prior to the GC-IRMS analysis (e.g., Engel et al. 1990; Silfer et al. 1991; Demmelmair and Schmidt 1993; Merritt and Hayes 1994; Metges et al. 1996; Chikaraishi et al. 2010a). However, this technique is still in developmental stages particularly for carbon isotope analysis of amino acids due to associated methodological challenges (e.g., Metges and Daenzer 2000; Docherty et al. 2001; Corr et al. 2007a, 2007b; Chikaraishi and Ohkouchi 2010). During the derivatization, the acylation of the amino group generates an artificial isotopic fractionation (εa) on the carbonyl-carbon of acyl moiety in the derivatives, because the carbon atom in derivatization reagents bond with amino acids in a non-quantitative reaction (Silfer et al. 1991; Rieley 1994; Metges and Daenzer 2000; Docherty et al. 2001; Chikaraishi and Ohkouchi 2010). This results in three methodological issues: (1) amino acids have large diversity in the structural characteristic (e.g., carbon skeleton, functional group, reaction points, polarity, etc.), resulting in diverse kinetic isotope effects; (2) the εa value are strongly dependent on the molar balance between amino acids and derivative reagents; and (3) the εa values are driven by the molar balance among amino acids, as well as on the sample matrix (particularly the content of hydroxyl groups) (Chikaraishi and Ohkouchi 2010). These issues can cause unreliable determination of the δ13CAA values by GC-IRMS analysis (e.g., Silfer et al. 1991; Chikaraishi and Ohkouchi 2010; Dunn et al. 2011). Even though the issues of isotope effect and molar balance of given amino acids can be calculated and controlled (Corr et al. 2007a, 2007b), the control (or correction) of the εa values derived from the contribution of matrix is difficult to ascertain in many cases (Chikaraishi and Ohkouchi 2010). For instance, there is a high dissimilarity in the PFC composition between plants (i.e., cellulose-based) and herbivore (i.e., protein-based) tissues. This results in a large disparity of the εa values for individual amino acids between samples derived from producers and herbivores (Chikaraishi and Ohkouchi 2010; Dunn et al. 2011). To avoid these methodological issues concerning the δ13CAA measurement, we used four carefully selected animal–animal combinations such that the molar balance of amino acids is comparable across consumers and diets, and this was later confirmed by direct quantification (Table 1, Fig. 1). Furthermore, each combination was designed such that the sample matrixes are comparable across diet and consumer compartments. As a result, the uncertainty (i.e., precision) in the Δδ13CAA value obtained from the analysis of each combination was better than 0.8–3.3‰, the propagation of analytical precision for δ13C measurement being 0.7–1.4‰.

Trophic isotopic discrimination (Δδ15N and Δδ13C) of amino acids

Based on the isotopic discriminations observed, the seven amino acids investigated are classified into the following three groups (Fig. 2): (1) no significant fractionation, with near-zero values of both Δδ15N and Δδ13C values for the aromatic amino acid (i.e., phenylalanine); (2) a small positive fractionation of the Δδ15N values, but no fractionation of the Δδ13C values for the branched-chain amino acids (i.e., valine, leucine, and isoleucine); and (3) a large positive fractionation for both Δδ15N and Δδ13C values for the other amino acids (i.e., glycine, alanine, and glutamic acid). Trends in the isotopic discrimination for the first two groups in the present study are consistent with those reported in previous studies (e.g., Chikaraishi et al. 2009; McMahon et al. 2010, 2015; Steffan et al. 2015). On the other hand, we found positive Δδ13C values in the last group, which is consistent with the values found in McMahon et al. (2015). However, several previous studies reported a broad range of positive and negative values for the Δδ13C values (e.g., Fantle et al. 1999; Howland et al. 2003; McMahon et al. 2010). Although specific reasons for such conflicting results are unknown, they may be attributed to the methodological issues concerning the carbon isotopic composition of amino acids.

Physiological mechanisms for the isotopic discrimination (Δ)

The kinetic isotopic fractionation (ε) within organic compounds is principally controlled by the isotope effect (α) and flux (F) of the processes that either form or cleave chemical bonds in metabolic pathways of organisms (e.g., Hoering 1957; Mariotti et al. 1981; Hayes 2001; Chikaraishi 2014; Ohkouchi et al. 2015; Goto et al. 2018). In the case of nitrogen, the Δδ15NAA values in consumer–diet combination can be explained by the activity of deamination for the “source” (e.g., phenylalanine) and “trophic” (e.g., valine, leucine, isoleucine, alanine, and glutamic acid) amino acids, respectively (Fig. 3a) (Chikaraishi et al. 2009, 2010b; Steffan et al. 2015; McMahon and McCarthy 2016; Ohkouchi et al. 2017). Consistency of the Δδ15NAA values between this and previous studies further validates this traditional interpretation.

Fig. 3
figure3

Simplified metabolic map of degradation and synthetic networks of the amino acids (i.e., glycine, alanine, valine, leucine, isoleucine, glutamic acid, and phenylalanine) with respect to isotopic fractionation: a 15N/14N associated with deamination of amino acids and b 13C/12C associated with decarboxylation of pyruvic acid (Pyr) and α-ketoglutaric acid (α-Kg). 15N can be enriched in the six amino acids (i.e., glycine, alanine, valine, leucine, isoleucine, and glutamic acid), and 13C can be enriched in the three amino acids (i.e., glycine, alanine, and glutamic acid) and their precursors on the pathway

The Δδ13CAA values in consumer–diet combination have been insufficiently understood in previous studies. Four decades ago, DeNiro and Epstein (1977) first demonstrated that metabolic products (i.e., lipids) are greatly depleted in 13C (by 7–8‰) for E. coli cultured with glucose or pyruvic acid, but less depleted in 13C (only by 1‰) for that with acetate. This suggested that the carbon isotopic fractionation is closely related to the decarboxylation of pyruvic acid to form acetyl-CoA. They further proposed that the decarboxylation of pyruvic acid causes the depletion in 13C at the position of the carbonyl-carbon on acetyl-CoA, likely due to the preferential cleaving of the 12C-12C bond on the pyruvic acid in the decarboxylation (e.g., Monson and Hayes 1980, 1982; Melzer and Schmidt 1987: Chikaraishi 2014). Based on the findings from prior studies, we propose the following framework (Fig. 3b) to interpret the Δδ13CAA values observed in the present study:

  1. (1)

    The decarboxylation of pyruvic acid (Pyr) in glycolysis preferentially eliminates 12C as acetyl-CoA, leaving behind the enriched 13C in the residual pool of pyruvic acid.

  2. (2)

    Similarly, the decarboxylation of α-ketoglutaric acid (α-Kg) in the tricarboxylic acid (TCA) cycle preferentially eliminates 12C as succinyl-CoA (Suc-CoA), leaving behind the enriched 13C in the residual pool of α-ketoglutaric acid.

  3. (3)

    The enrichment of 13C in the residual pyruvic and α-ketoglutaric acids is eventually transferred to amino acids, when the consumers produce the amino acids through de novo synthesis (or metabolic routing) from these 13C-enriched residual acids.

According to these processes, the trophic discrimination of carbon isotopes (Δδ13C) is not found in “essential” amino acids, where synthetic flux is limited (i.e., phenylalanine, valine, leucine, and isoleucine in the present study). Since decarboxylation in glycolysis and TCA cycle elevates 13C content of the synthetic precursors of amino acids, the de novo synthesized nonessential amino acids incorporate the 13C into the newly produced amino acids in consumers, resulting in large Δδ13CAA values (i.e., glycine, alanine, and glutamic acid in the present study).

Key processes affecting the isotopic fractionation (ε)

From a theoretical perspective, the preferential “cleaving” of chemical bond between light isotopes (i.e., 12C-14N) compared to between light and heavy isotopes (i.e., 12C-15N and 13C-14N) of amino acids is the dominant process in the isotopic fractionation of amino acids in metabolic pathways (e.g., Chikaraishi et al. 2007, 2009). This leads to a pattern of simultaneous enrichment in 15N and 13C associated with deamination of amino acids. However, the results in the present study offer little support to this theoretical consideration and suggest that the Δδ15NAA values are independent of the Δδ13CAA values (particularly for branched-chain amino acids) in the studied consumers (Fig. 2). Therefore, we suggest that the isotopic fractionation (ε) is attributable to the alternative processes that involve the preferential “forming” of the 12C-14N or 12C-12C bond. Indeed, the deamination and decarboxylation initiate the formation of C-N and C-C bonds between substrate and enzyme, respectively, and ultimately cleave a chemical bond on the substrate. We therefore speculate that the “key” reactions responsible for the isotopic fractionation of amino acids proceeds in the following sequential pattern:

  1. (1)

    During “forming” of the C-N double bond between an amino acid and pyridoxal phosphate (PLP) at the initial step of deamination, 14N amino group in amino acids is preferentially bonded and deaminated, and this reaction leaves behind 15N in the residual pool of amino acids in consumer biomass (Fig. 4a).

  2. (2)

    During “forming” of the C-C bond between pyruvic acid (or α-ketoglutaric acid) and thiamine pyrophosphate (TPP) at the initial step of decarboxylation, the 12C α-carbon in pyruvic acid (or α-ketoglutaric acid) is preferentially bonded with the TPP and then decarboxylated from the neighboring carboxyl group carbon in pyruvic acid, resulting in that this reaction leaves behind 13C in the residual pool of pyruvic acid (or α-ketoglutaric acid) in consumer biomass (Fig. 4b). The enriched 13C can be transferred to those amino acids that are subsequently synthesized de novo by the consumers.

Fig. 4
figure4

a Possible scenarios of the isotopic fractionation during deamination of amino acids and b decarboxylation of pyruvic and α-ketoglutaric acids: the fractionation is caused by forming of C-N or C-C bond. Asterisk indicates atoms where isotopic fractionation occurs. Yellow highlight indicates reaction points accompanied with the isotopic fractionation

Implications

Compound-specific isotope analysis of amino acids has been widely used in food web ecology. While nitrogen isotopes have been used to estimate the trophic position (TP) of organisms, carbon isotopes have proven useful in tracing the producers at the foundations of food webs (reviewed in Ohkouchi et al. 2017). However, possible factors for trophic discrimination (Δ) and key reactions responsible for isotopic fractionation (ε) of amino acids cannot be sufficiently explained by isotopic fractionation caused by the cleaving of chemical bond. Moreover, artificial isotopic fractionation (εa) during the derivatization of amino acids increases uncertainty on the δ13CAA value observed.

Through careful selection of consumer–diet pairings, we were able to minimize the methodological error in the Δδ13CAA values, and delineate possible factors driving the isotopic discrimination and fractionation of amino acids (Table 3). The isotopic fractionation (ε) can be explained by proposed mechanisms involving the preferential formation of the 12C-14N bond (i.e., amino acids with PLP) and 12C-12C bond (i.e., amino acid precursors with TPP) (Fig. 4). In the case of nitrogen isotopes, although deamination of amino acids is a key reaction (Chikaraishi et al. 2007), the results in the present study further reveal that balance between degradation and synthetic flux is a dominant factor for controlling the trophic isotopic discrimination (Table 3). In the case of carbon isotopes, the results in the present study reveal that decarboxylation of the precursor molecules (e.g., pyruvic and α-ketoglutaric acids) is a key reaction, and that the resulting de novo synthetic flux is the dominant factor controlling trophic isotopic discrimination (Table 3). These key reactions form a biochemical intersection of amino acid metabolism, linking the isotope physiology of amino acids in organisms. We use a cross-plot between Δδ15N and Δδ13C to represent this intersection to classify amino acids into three groups (Table 3, Fig. 5):

  1. (1)

    Source-essential: The aromatic amino acid (i.e., phenylalanine) has the Δδ15N and Δδ13C values very close to zero, due to an absence of both deamination and synthesis activities in consumers (Fig. 5a).

  2. (2)

    Trophic-essential: The branched-chain amino acids (i.e., valine, leucine, and isoleucine) have high Δδ15N, but near-zero Δδ13C values, due to the presence of deamination and an absence of synthesis activities in consumers, respectively (Fig. 5b).

  3. (3)

    Trophic-nonessential: The other amino acids (i.e., glycine, alanine, and glutamic acid) have an inverse correlation between the Δδ15NAA and Δδ13CAA values, due to varying degrees of involvement in deamination and synthesis in consumers (Fig. 5c).

Table 3 Possible factors for controlling trophic discrimination (Δ) and key reactions responsible for isotopic fractionation (ε) on consumers
Fig. 5
figure5

Possible classification of amino acids: a source-essential, b trophic-essential, and c trophic-nonessential on the cross-plot of the Δδ15N and Δδ13C values

The diversity and variation in the trophic isotopic discrimination among amino acids are closely related to physiological status of organisms. Balance between degradation and synthesis of amino acids shifts based on the energy requirement with respect to environmental conditions such as satiation versus starvation, PFC balance in diets, and life strategy specific to organisms (Germain et al. 2013; Chikaraishi et al. 2015; McMahon et al. 2015; Steffan et al. 2013, 2015; Takizawa et al. 2017; Choi et al. 2018).

The proposed bridged perspective between the Δδ15NAA and Δδ13CAA values can reveal the potential processes affecting the isotopic compositions of ecological and environmental samples. While the near-zero values of Δδ13C of essential amino acids (e.g., phenylalanine, valine, leucine, and isoleucine) have been used to characterize the contribution from basal resources to ecosystems, the broader basis of such empirical data remained largely unknown (Larsen et al. 2009, 2013, 2015; McMahon et al. 2010, 2016). Our findings suggest that such observation arises from the fact that essential amino acids are largely diet-derived, leaving limited scope for the incorporation of decarboxylation-derived 13C-enriched precursor molecules (Fig. 2). Additionally, there is little information explaining the idiosyncratic behavior of specific amino acids. For instance, there is a large variation in the Δδ15N value of glycine (e.g., 4.0 ± 3.5‰ in Chikaraishi et al. 2010b, and 3.9 ± 4.9 in McMahon and McCarthy 2016), and the Δδ15N values of glutamic acid have been frequently compressed in several animals, particularly for high trophic level consumers that have urea/uric acid cycle (e.g., McMahon and McCarthy 2016). The inverse relationship with respect to Δδ15NAA and Δδ13CAA values for trophic-nonessential amino acids, glycine, alanine, and glutamic acid, observed in the present study can potentially explain this significantly large variation. Increasing frequency of deaminating reactions expands the Δδ15NAA values, and lowered influx of de novo-synthesized amino acids compresses the Δδ13CAA values. Conversely, increased abundance of de novo-synthesized amino acids expands Δδ13CAA values, and this is accompanied by the concomitant compression of the Δδ15NAA values. The combined analysis of the Δδ15N and Δδ13C values of individual amino acids can thus reflect the metabolic status of consumers and offer a more holistic view of trophic interactions.

The main limitation associated with δ13CAA and Δδ13CAA analysis (e.g., Chikaraishi and Ohkouchi 2010) arises from the artificial isotopic fractionation (εa in Eqs. 2 and 3) introduced during amino acids derivatization. This methodological challenge has limited the scope of empirical quantification of isotopic fractionation (ε) associated with “key” processes in metabolism. Continual efforts directed towards method development (e.g., Metges and Daenzer 2000; Docherty et al. 2001; Corr et al. 2007a, 2007b; Smith et al. 2009; Chikaraishi and Ohkouchi 2010) will improve the utility of amino acid isotope approaches in the biogeoscience.

Availability of data and materials

The data for this paper are tabulated in this manuscript.

Abbreviations

AA:

Amino acid

DNA:

Deoxyribonucleic acid

AIR:

Atmospheric nitrogen

Pv/iPr derivatization:

Pivaloyl/isopropyl derivatization

GC/IRMS:

Gas chromatography/isotope ratio mass spectrometry

GC-IRMS:

Gas chromatograph-isotope ratio mass spectrometer

VPDB:

Vienna Peedee Belemnite

SS:

Sea slug–sponge

LA:

Ladybug beetle–aphid

GF:

Green lacewing–fall armyworm

GG:

Green lacewing–green lacewing

PFC:

Protein–fat–carbohydrate

Pyr:

Pyruvic acid

3PG:

3-Phosphoglyceric acid

PEP:

Phosphoenolpyruvic acid

α-Kg:

α-Ketoglutaric acid

Suc-CoA:

Succinyl-CoA

PLP:

Pyridoxal phosphate

TCA:

Tricarboxylic acid

TPP:

Thiamine pyrophosphate

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Acknowledgements

We greatly appreciate Dr. Eitaro Wada for his lectures, expert comments/advices, and constructive discussion, which deconvoluted complex issues in the study of stable isotope physiology and ecology. We also appreciate Dr. Naoto F. Ishikawa (JAMSTEC), Prof. Rong Fan (Northwest University), and Mr. Daochao Xing (Hokkaido University) for the discussion of isotope ratios in amino acids and those ecological aspects. Two anonymous reviewers are greatly acknowledged for improving the manuscript. We thank to the young researcher travel grant (Y. Takizawa) of the Biogeoscience section (FY2017) in Japan Geoscience Union (JpGU), which gave us an opportunity to write this paper in Progress in Earth and Planetary Science.

Funding

This work was supported by JSPS KAKENHI Grant Number JP 16 J01704 to Y. Takizawa, Grant-in-Aid for Scientific Research of the JSPS to Y. Chikaraishi (No. 17H02988), Y. Takano (No. 16H04083), and N. Ohkouchi (No. 16H02236), USDA-ARS appropriated funds to S.A. Steffan, as well as funds from Silicone Industry Association of Japan to Y. Chikaraishi.

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Y.Takizawa, Y.Takano, and Y.C. conceived the present study. Y.C. collected natural samples and S.A.S. provided reared samples. The manuscript was written primarily by Y.Takizawa with contribution from Y.C., B.C., P.S.D., S.A.S., Y.Takano, N.O., and N.O.O. Y.Takizawa and Y.C prepared and analyzed amino acid derivatives. The authors read and approved the final manuscript.

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Correspondence to Yuko Takizawa.

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Takizawa, Y., Takano, Y., Choi, B. et al. A new insight into isotopic fractionation associated with decarboxylation in organisms: implications for amino acid isotope approaches in biogeoscience. Prog Earth Planet Sci 7, 50 (2020). https://doi.org/10.1186/s40645-020-00364-w

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Keywords

  • Compound-specific isotope analysis
  • Amino acids
  • Nitrogen
  • Carbon
  • Trophic discrimination
  • Deamination
  • Decarboxylation
  • Degradation
  • Synthesis
  • Isotope physiology