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Progress in Earth and Planetary Science
Fig. 4 | Progress in Earth and Planetary Science

Fig. 4

From: Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis

Fig. 4

a Structure of coenzyme factor 430 (F430), which has a chemical formula of C42H51N6NiO13. F430 is a hydrocorphinoid nickel complex that functions as an active site in MCR. The MCR–F430 complex catalyzes the last step of methanogenesis involving the reduction of methyl coenzyme M into CH4. b, c Chromatograms of the planktonic cyanobacterial samples analyzed via LC/ESI–MS/MS in MRM mode (Kaneko et al. 2014). The F430M product ion was set to m/z 844.3. The concentration of native F430 with assessment of the F430 epimer ratio enabled us to estimate the biomass of the in situ living methanogenic archaea (Kaneko et al. 2021)

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