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Fig. 6 | Progress in Earth and Planetary Science

Fig. 6

From: Accessing the energy-limited and sparsely populated deep biosphere: achievements and ongoing challenges of available technologies

Fig. 6

Adopted from Morono et al. (2020)

Detection of substrate incorporation into microbial cells revived in 101.5 Ma sediment. Cells from incubations with 13C-bicarbonate and 15N ammonium (a, b, c, d) and 13C,15N-Amino acid mix (e, f, g, h) are shown. a, e SYBR Green I-stained cells under fluorescence microscopy. b, c, f, g Ratio images of 13C/12C (b, f) and 12C15N/12C14N (c, g) ratios of the same regions imaged in a and e, demonstrating locations of 13C and 15N incorporation. Color scale range of the ratios are shown as number appearing at top and bottom of the color bar. The background membrane region, which is identified by fluorescence images, is excluded from the ratio calculation and shown as black background. d, h Secondary electron (NanoSIMS) images of the same region in a and e. Bars represent 5 µm.

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