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Fig. 3 | Progress in Earth and Planetary Science

Fig. 3

From: Accessing the energy-limited and sparsely populated deep biosphere: achievements and ongoing challenges of available technologies

Fig. 3

Adopted from Heuer et al. (2020)

SYBR Green I staining of vegetative cells (a) and endospores (b) of Bacillus subtilis NBRC13719. This figure illustrates the difficulty in identifying spores even of pure cultures of organisms that are mixed into sterilized sediments. In (c), endospores were mixed in sterilized sediment prior to staining by SYBR Green I. Several representative yellowish endospores, which are difficult to distinguish from sediment particles, are indicated by white arrows as examples. Bars represent 10 μm.

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