Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis

Progress in Earth and Planetary Science

Table 4 PCR primers for archaeal 16S rRNA and mcrA genes for the present initial report

Target	Primer name	Direction	Sequences (5′ → 3′)	Hybridization temperature (°C)	References
Archaeal 16S rRNA gene	340F	Forward	CCCTAYGGGGYGCASCAG	52	Gantner et al. (2011)
Archaeal 16S rRNA gene	1000R	Reverse	GGCCATGCACYWCYTCTC	52	Gantner et al. (2011)
mcrA gene	Luton-mcrA F	Forward	GGTGGTGTMGGATTCACACARTAYGCWACAGC	50	Luton et al. (2002)
mcrA gene	Luton-mcrA R	Reverse	TTCATTGCRTAGTTWGGRTAGTT	50	Luton et al. (2002)
mcrA gene	MCR F	Forward	TAYGAYCARATHTGGYT	50	Springer et al. (1995)
mcrA gene	MCR R	Reverse	ACRITCATNGCRTARTT	50	Springer et al. (1995)
mcrA gene	ME 3F	Forward	ATGTCNGGTGGHGTMGGSTTYAC	55	Sørensen et al. (2009)
mcrA gene	ME 2R	Reverse	TCATBGCRTAGTTDGGRTAGT	55	Sørensen et al. (2009)
mcrA gene	mlas	Forward	GGTGGTGTMGGDTTCACMCARTA	55	Steinberg and Regan (2008)
mcrA gene	mcrA-rev	Reverse	CGTTCATBGCGTAGTTVGGRTAGT	55	Steinberg and Regan (2008)