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Table 3 PCR amplification of the archaeal 16S rRNA and mcrA genes. The PCR products were loaded onto a 1.2% agarose gel with negative and positive controls and a DNA size standard

From: Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis

Target

Archaeal 16S rRNA gene

mcrA gene

PCR primer set

340F/1000R

Luton-mcrA F/R

MCR F/MCR R

ME 3F/ME 2R

mlas/mcrA-rev

Planktonic cyanobacteria

(September 2018)

 − 

 − 

 − 

 − 

 − 

Sediment 0–10 cm depth

(September 2018)

 + 

 + 

 − 

 + 

 + 

  1. The presence or absence of the PCR product is indicated as ( +) and ( −), respectively. The annealing temperature for each primer set is listed in Table 4