Table 1 Results of molecular cloning for the environmental DNA from five sites
From: Phylogenetic positions of “pico-sized” radiolarians from middle layer waters of the tropical Pacific
Site | Depth (m) | CTD information | Volume of filtered seawater (L) | DNA (ng/μl) | PCR | Clone number | Spumellarian clones | |
|---|---|---|---|---|---|---|---|---|
A | 1000 | 5 | A | 2.6 | − | |||
5 | B | 183.4 | − | |||||
A | 400 | 5 | A | 47.0 | − | |||
5 | B | 56.5 | − | |||||
A | 120 | Chlorophyll max | 5 | A | 77.9 | + | 0 | |
5 | B | 48.3 | − | |||||
C | 750 | 5 | A | 94.0 | − | |||
5 | B | 197.0 | − | |||||
C | 400 | 5 | A | 113.8 | + | 5 | 3 | |
5 | B | 96.1 | − | |||||
C | 150 | 5 | A | 79.7 | + | 0 | ||
5 | B | 57.0 | − | |||||
C | 100 | Chlorophyll max | 5 | A | 64.3 | + | 0 | |
5 | B | 100.8 | − | |||||
D | 400 | 5 | A | 50.8 | − | |||
5 | B | 22 | − | |||||
D | 250 | 5 | A | 58.6 | + | 11 | 2 | |
5 | B | 15.8 | + | 18 | 1 | |||
D | 120 | 5 | A | 78.4 | + | 0 | ||
5 | B | 23.7 | − | |||||
D | 90 | Chlorophyll max | 5 | A | 39.9 | + | 7 | 0 |
5 | B | 33 | + | 8 | 0 | |||
E | 400 | 5 | A | 74.0 | − | |||
5 | B | 31.5 | − | |||||
E | 150 | 5 | A | 62.8 | − | |||
5 | B | 18.0 | − | |||||
E | 105 | 5 | A | 47.0 | + | 0 | ||
5 | B | 30.4 | − | |||||
E | 70 | Chlorophyll max | 5 | A | 53.1 | − | ||
5 | B | 39.5 | − | |||||
F | 400 | 5 | A | 5.6 | − | |||
5 | B | 15.6 | − | |||||
F | 200 | 5 | A | 89.1 | − | |||
5 | B | 60.6 | − | |||||
F | 130 | Chlorophyll max | 5 | A | 93.9 | − | ||
5 | B | 23.4 | − | |||||