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Table 3 Radio-isotope-tracer carbon assimilation of microbial community in the CORK fluids and positive controls

From: Cool, alkaline serpentinite formation fluid regime with scarce microbial habitability and possible abiotic synthesis beneath the South Chamorro Seamount

Cruise ID

HPD dive#

Tracer and incubation condition

Incubation time (days)

Net assimilation (nmol day−1 L−1)

NT09-01

943

14C-CH4 (0.25 MBq per bottle and 0.4 mM)

54

0.036

943

14C-CH4 (0.25 MBq per bottle and 0.4 mM) with 40 μM O2

54

0.049

NT09-01

947

14C-Leucine (0.25 MBq per bottle and 0.1 mM)

52

0.014

947

14C-Formate (0.25 MBq per bottle and 0.1 mM)

52

0.06

947

14C-Acetate (0.25 MBq per bottle and 0.1 mM)

52

0.004

NT09-01

948

14C-CH4 (0.25 MBq per bottle and 0.4 mM) with 10 mM NaSO4 and 0.5 mM Na2S

49

0.039

948

14C-CO (0.25 MBq per bottle and 0.4 mM)

49

n.d.a

NT09-07

1007

14C-CH4 (0.25 MBq per bottle and 0.4 mM)

123

0.2

1007

14C-HCO3− (0.25 MBq per bottle and 0.1 mM) with 0.4 mM H2

123

1.4

1009

14C-Formate and 14C-acetate (each of 0.25 MBq per bottle and 0.1 mM)

122

0.05

An aerobic methanotroph culture

14C-CH4 (0.25 MBq per bottle and 0.4 mM)

1

20

An autotrophic community in diffusing hydrothermal fluid

14C-HCO3− (0.25 MBq per bottle and 0.1 mM)

5

2.3

  1. an.d. not detected