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Table 3 Radio-isotope-tracer carbon assimilation of microbial community in the CORK fluids and positive controls

From: Cool, alkaline serpentinite formation fluid regime with scarce microbial habitability and possible abiotic synthesis beneath the South Chamorro Seamount

Cruise ID HPD dive# Tracer and incubation condition Incubation time (days) Net assimilation (nmol day−1 L−1)
NT09-01 943 14C-CH4 (0.25 MBq per bottle and 0.4 mM) 54 0.036
943 14C-CH4 (0.25 MBq per bottle and 0.4 mM) with 40 μM O2 54 0.049
NT09-01 947 14C-Leucine (0.25 MBq per bottle and 0.1 mM) 52 0.014
947 14C-Formate (0.25 MBq per bottle and 0.1 mM) 52 0.06
947 14C-Acetate (0.25 MBq per bottle and 0.1 mM) 52 0.004
NT09-01 948 14C-CH4 (0.25 MBq per bottle and 0.4 mM) with 10 mM NaSO4 and 0.5 mM Na2S 49 0.039
948 14C-CO (0.25 MBq per bottle and 0.4 mM) 49 n.d.a
NT09-07 1007 14C-CH4 (0.25 MBq per bottle and 0.4 mM) 123 0.2
1007 14C-HCO3 (0.25 MBq per bottle and 0.1 mM) with 0.4 mM H2 123 1.4
1009 14C-Formate and 14C-acetate (each of 0.25 MBq per bottle and 0.1 mM) 122 0.05
An aerobic methanotroph culture 14C-CH4 (0.25 MBq per bottle and 0.4 mM) 1 20
An autotrophic community in diffusing hydrothermal fluid 14C-HCO3 (0.25 MBq per bottle and 0.1 mM) 5 2.3
  1. an.d. not detected