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Table 2 Initial stable hydrogen isotopic conditions for the hydrogenotrophic methanogenesis experiments

From: Hydrogen and carbon isotope systematics in hydrogenotrophic methanogenesis under H2-limited and H2-enriched conditions: implications for the origin of methane and its isotopic diagnosis

Batches Isotope labeling Initial value \( {\updelta}^{\mathrm{eq}}{\mathrm{D}}_{{\mathrm{H}}_2\hbox{--} {\mathrm{H}}_2\mathrm{O}} \)
D2O DH \( {\updelta \mathrm{D}}_{{\mathrm{H}}_2\mathrm{O}} \) \( {\updelta \mathrm{D}}_{{\mathrm{H}}_2} \)
Thermophilic coculture experimental set (TC, at 55 °C)
A1 −58
A2 −62
A3 + +1624
A4 + +1639
A5 ++ +3771
A6 ++ +3787
Mesophilic coculture experimental set (MC, at 25 °C)
B1 −62
B2 −58
B3 + +1406
B4 + +1413
B5 ++ +3662
B6 ++ +3286
Thermophilic pure culture experimental set (TP, at 60 °C)  
C1 −59 −358 DH-rich
C2 + −53 +330 DH-rich
C3 + +1097 −287 Equilibrated
C4 + ++ +1210 +1886 DH-rich
C5 ++ +2574 −350 D2O-rich
C6 ++ + +2317 +70 Equilibrated
Mesophilic pure culture experimental set (MP, at 30 °C)  
D1 −54 −344 DH-rich
D2 + + +1427 +145 DH-rich
D3 + + +1424 +22 DH-rich
D4 + +1369 −350 Equilibrated
D5 + +1382 −349 Equilibrated
D6 ++ +3096 −351 D2O-rich
D7 ++ + +3142 +85 Equilibrated