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Progress in Earth and Planetary Science
Fig. 1 | Progress in Earth and Planetary Science

Fig. 1

From: Hydrogen and carbon isotope systematics in hydrogenotrophic methanogenesis under H2-limited and H2-enriched conditions: implications for the origin of methane and its isotopic diagnosis

Fig. 1

Temporal changes in H2, CH4, and CO2 in four culture experimental sets. The experimental sets for the thermophilic coculture (a–f; TC), the mesophilic coculture (g–l; MC), the thermophilic pure culture (m–r; TP), and the mesophilic pure culture (s–x; MP) consisted of experimental batches A1–A6, B1–B6, C1–C6, and D1–D7, respectively. The pH2 values (panels a, g, m, and s), the pCH4 values (panels b, h, n, and t), the \( {\updelta \mathrm{D}}_{{\mathrm{H}}_2} \) values (panels c, i, o, and v), the \( {\updelta \mathrm{D}}_{{\mathrm{CH}}_4} \) values (panels d, j, p, and v), the \( {\updelta}^{13}{\mathrm{C}}_{{\mathrm{C}\mathrm{O}}_2} \) values (panels e, k, q, and w), and the \( {\updelta}^{13}{\mathrm{C}}_{{\mathrm{C}\mathrm{H}}_4} \) values (panels f, l, r, and x) were determined for a certain interval of growth from immediately after inoculation to the stationary phase of growth. The symbols are common within each experimental set (TC, MC, TP, and MP)

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